Last edited by Samujora
Monday, July 27, 2020 | History

2 edition of Growth of metabolically active HepG2, human hepatoma, cells in high density cultures found in the catalog.

Growth of metabolically active HepG2, human hepatoma, cells in high density cultures

Russell Mark Traynor

Growth of metabolically active HepG2, human hepatoma, cells in high density cultures

by Russell Mark Traynor

  • 260 Want to read
  • 22 Currently reading

Published .
Written in English


Edition Notes

Thesis (M.Sc.) - University of Surrey, 1996.

StatementRussell Mark Traynor.
ContributionsUniversity of Surrey. School of Biological Sciences.
ID Numbers
Open LibraryOL19356352M

Hepatoma-derived growth factor (HDGF) is a hepa- rin-binding protein, which has been purified from the conditioned media of HUH-7 hepatoma cells. 16,17 HDGF stimulates the proliferation of fibroblasts, endothelial cells, vascular smooth muscle cells, and some hepatoma cells, including HuH Its primary sequence has noCited by: Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health.

  The H6 hepatoma cell line did take some time to grow out. We used to thaw in T25s - RPMI+10%FBS, pen-strep, b-ME, HEPES, glutamine - usually about days with a couple of medium changes (but no scrapping off of cells). Don't know if . Vitamin B6 suppresses growth and expression of albumin gene in a human hepatoma cell line HepG2 Nutrition and Cancer, Vol. 28, No. 2 Alteration of secretion of parathyroid hormone-related peptide and expression of its mRNA in a human hepatoma cell line (HEP G2) Cited by:

Unlike other cell lines (e.g., HepG2 and Fa2N-4) HepaRG cells have expression levels of multiple functional Phase 1 and 2 drug metabolizing enzymes (DME) and nuclear receptors consistent with levels observed in primary human hepatocytes, and therefore, are more suitable to assess the metabolic stability of candidate compounds (Figure 2). nutrients Article Identification of the Secreted Proteins Originated from Primary Human Hepatocytes and HepG2 Cells Andras Franko 1,2,3,*, Sonja Hartwig 3,4, Jörg Kotzka 3,4, Marc Ruoß 5, Andreas K. Nüssler 5, Alfred Königsrainer 6, Hans-Ulrich Häring 2,3,7, Stefan Lehr 3,4,y and Andreas Peter 1,2,3,y 1 Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Author: Andras Franko, Sonja Hartwig, Jörg Kotzka, Marc Ruoß, Andreas K. Nüssler, Alfred Königsrainer, Hans-.


Share this book
You might also like
Hidden power

Hidden power

church and clinical pastoral education

church and clinical pastoral education

simulation of the control functions of the mammalian heart.

simulation of the control functions of the mammalian heart.

American car spotters guide, 1940-1965.

American car spotters guide, 1940-1965.

non-dramatic works of Thomas Dekker

non-dramatic works of Thomas Dekker

Unsteady heat and mass transfer in helical tube bundles

Unsteady heat and mass transfer in helical tube bundles

Properties of concrete.

Properties of concrete.

Sociology In Our Times

Sociology In Our Times

Use made of Current Papers in Physics

Use made of Current Papers in Physics

D-NURBS: dynamic non-uniform rational B-splines

D-NURBS: dynamic non-uniform rational B-splines

The Origin and History of Irish Names of Places, Set

The Origin and History of Irish Names of Places, Set

Pastel Painters Pocket Palette

Pastel Painters Pocket Palette

Court house and post office at Rutland, Vermont

Court house and post office at Rutland, Vermont

Licensing intellectual property

Licensing intellectual property

Gillingwaters history of Lowestoft, re-written and arranged in modern style, with additions by various authors,and brought up to date.

Gillingwaters history of Lowestoft, re-written and arranged in modern style, with additions by various authors,and brought up to date.

Growth of metabolically active HepG2, human hepatoma, cells in high density cultures by Russell Mark Traynor Download PDF EPUB FB2

Cultures of primary hepatocytes and hepatoma cell line HepG2 are frequently used in in vitro models for human biotransformation studies. In this study, we characterized and compared the capacity of these model systems to indicate the presence of different classes of promutagens.

Genotoxic sensitivity, enzyme activity, and gene expression were monitored in response to treatment with food. Despite its increasing use, the growth, functional status and response of this cell line in high density cell cultures have not been well characterised in the literature. In this paper we describe the morphological, growth and biochemical responses of HepG2 cells to the glucocorticoid by: 1.

HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions. In this chapter, freezing, thawing, and subculturing procedures for HepG2 cells are by: Busch SJ, et al.

Differential regulation of hepatic triglyceride lipase and 3-hydroxy methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2.

Biol. Chem.PubMed: Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis Article in Biochimica et Biophysica Acta (11) December with 73 Reads.

High glucose is toxic to HepG2 can grow in DMEM with mM glucose /MEM with mM glucose makes it resiatant to glucose similar to insulin resistance. Cite 20th Dec, HepG2 Human hepatoma Line Origin and Characteristics.

HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a year-old Caucasian male who had a well-differentiated hepatocellular carcinoma, which is the fifth most-common cancer worldwide.

The HepG2 cell line is commonly used in drug metabolism and hepatoxicity studies. Different originations of HepG2 and Hep3B cells. HepG2 and Hep3B were originally established by Aden et al. ().They were isolated from liver biopsy specimens of a year-old Caucasian male from Argentina with primary HB, or an 8-year-old black male from the US with primary HCC (Aden et al.

; Knowles et al. ), cell lines contain distinctive rearrangements of Cited by: Cultures of primary hepatocytes and hepatoma cell line HepG2 are frequently used in in vitro models for human biotransformation studies.

In this study, we characterized and compared the capacity of these model systems to indicate the presence of different classes of promutagens. Genotoxic sensitivity, enzyme activity, and gene expression were monitored in response to treatment with food.

The cells will respond to stimulation with human growth hormone. Application Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric.

Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived from the liver tissue of a year-old African American adolescent boy with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

The cells secrete a variety of major plasma. Fig. mRNA expression for haptoglobin, albumin and aldolase B. Transcripts were measured in primary human hepatocyte cultures, confluent HepG2 cells and HepaRG cells after different times of culture and maintained after day 15 in the absence or presence of 2% DMSO.

The values are expressed as percentages compared to a pool of three different Cited by: Fucoxanthin inhibited the growth of human hepatoma cells through cell cycle arrest at G 0 /G 1 phase. (A) HepG2 cells were treated with the indicated concentration of fucoxanthin or % tetrahydrofuran (THF) as the vehicle control for different time by: Human hepatocytes are the gold standard for toxicological studies but they have several drawbacks, like scarce availability, high inter-individual variability, a short lifetime, which limits their applicability.

The aim of our investigations was to determine, whether HepaRG cells could replace human hepatocytes in uptake experiments for toxicity studies. HepaRG is a hepatoma cell line with. Alternatives include the HepG2‐derivative C3A cells selected as a more differentiated and metabolically active hepatic phenotype.

Human HepaRG cells are an alternative organotypic co‐culture model of hepatocytes and cholangiocytes reported to maintain in vivo ‐like liver‐specific functions, including intact Phase I–III drug by: Hepatoma‐derived growth factor (HDGF) is a novel mitogen for hepatocytes.

The mRNA and protein levels of HDGF are up‐regulated in malignant hepatoma Cited by: HepG2 hepatoma. The HepG2 cell line is a human hepatoma commonly used to study the cellular responses upon infection with hepatotropic pathogens or upon activation of the aryl hydrocarbon receptor (AhR) pathways by environmental pollutants or diet-derived compounds.

HepG2 culture conditions Medium: DMEM + 10% FBS + 1% pen-strep. Given the there are many formulations of DMEM and different versions of serum, we would like to provide the catalog numbers of the stuff that we are using: DMEM - HyClone cat.

SH & serum - HyClone cat. SH Both could be purchased from VWR. Procedure: Size: 11KB. differentiated cells.

Accordingly, it was found that only human hepatocyte primary cultures were susceptible to HBV infection (1–4). However, the use of this model is hampered by the limited availability and the inherent variability of human liver material. Even though several human hepatoma-derived cell Cited by: 3.

Transfer the cells into the Growth medium and centrifuge at rpm for 5min. Resuspend the cell pellet in an appropriate amount of fresh growth medium. Incubate the cells at 37°C in a 5% CO2 in air atmosphere incubator.

Change the fresh growth medium every 2 to 3 days. Subculture Subculture cells until density reaches 70‐80%. The molecular mechanism of the HGF-induced growth inhibition of tumor cells remains obscure.

We have investigated the intracellular signaling pathway involved in the antiproliferative effect of HGF on the human hepatocellular carcinoma cell line HepG2. HGF .growth arrest of human hepatoma HepG2 cells [14]. However, whether there is any miRNA involved in PKCα-mediated cell growth arrest is still unknown.

MiR was shown to promote apoptosis and suppress FOS oncogene expression in human hepatoma cells and to act as tumor suppressor gene in carcinogenesis of human hepatoma [15,16].Cited by: nutrients Article Chemopreventive Activities of Sulforaphane and Its Metabolites in Human Hepatoma HepG2 Cells Peng Liu 1, Wei Wang 1, Zhigang Zhou 1, Andrew J.

O. Smith 2, Richard P. Bowater 2 ID, Ian Michael Wormstone 2, Yuqiong Chen 3 and Yongping Bao 1,* ID 1 Norwich Medical School, University of East Anglia, Norfolk, Norwich NR4 7UQ, UK; [email protected] (P.L.); Cited by: 3.